RESUMO
We have developed a modified cyclodextrin solid (MCS) medium using the selective antibiotic cefdinir. MCS medium exhibited higher sensitivity (95.6%; any culture-positive sample as reference) and greater inhibition of nasopharyngeal flora than did Bordet-Gengou agar (65.2%, P = 0.009) or cyclodextrin solid medium (39.1%, P < 0.001).
Assuntos
Antibacterianos , Bordetella pertussis , Cefalosporinas , Meios de Cultura/química , Ciclodextrinas , Nasofaringe/microbiologia , Ágar , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Técnicas Bacteriológicas , Bordetella pertussis/crescimento & desenvolvimento , Bordetella pertussis/isolamento & purificação , Candida albicans/efeitos dos fármacos , Cefdinir , Cefalosporinas/farmacologia , Criança , Contagem de Colônia Microbiana , Humanos , Sensibilidade e Especificidade , Especificidade da EspécieRESUMO
We determined antimicrobial susceptibilities and analyzed molecular epidemiology of 26 strains of Bordetella pertussis clinically isolated and then performed pulsed-field gel electrophoresis (PFGE) in Japan (Japanese Pertussis Surveillance Group Participants), from 2001 to 2002. The MICs of erythromycin, clindamycin, tetracyclines, fluoroquinorones, trimethoprim-sulfamethoxazole and rifampicin of all isolates against these showed 1 microgram/ml or less. Sparfloxacin is the most potent agent, of which the MICs showed 0.008-0.016 microgram/ml. Results of DNA fingerprinting by pulsed-field gel electrophoresis (PFGE) differentiated three types (Type I; 11 strains (42%), type II; 14 strains (54%) and type III; 1 strains (4%)). However, no relation between regions and identical PFGE patterns was found in this study. Further, surveillance of the antimicrobial susceptibilities and molecular epidemiology of B. pertussis will be required.